Measurement of apoptosis in long-term cultures of human ovarian tissue

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I-16: Assessment of The Vitrified Ovarian Tissue in Long Term Culture

In vitro culture of human ovarian tissue the following cryopreservation is proposed for follicular development. There are no techniques that guarantee successful maturation of the follicles within the excised tissue. The viability of cultured human ovarian tissue improved by adding some growth factors to the culture media. The efficiency of vitrification as the cryopreservation method for human...

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O-4: Morphological Analyses and Apoptosis Genes Expression Evaluation in Vitrified Human Ovarian Tissue after Warming, Long Term Culturing and Xenotransplantation

Background In vitro culturing and retransplantion of vitrified- warmed ovarian tissue are two ways to restore fertility after radiation or chemotherapy.This study aimed to evaluate the incidence of apoptosis in vitrified human ovarian tissue after warming, long term culturing and xenotransplantation by morphological analyses and apoptosis genes expression evaluation. MaterialsAndMethods We obta...

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Human Ovarian Tissue Vitrification/Warming Has Minor Effect on the Expression of Apoptosis-Related Genes

Background: In this study, we evaluated the incidence of apoptosis at the ultrastructural levels and expression of some apoptosis-related genes in vitrified human ovarian tissue just after warming. Methods: Human ovarian tissue biopsies from 23 women after caesarean section were transported to the laboratory within 2 hours, and then they were cut into small pieces. Some pieces were vitrified an...

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Spontaneous Mesenchymal to Epithelial Like Tissue Transition (MET) in a Long Term Human Skin Culture

In an attempt to isolate multipotent stem cells from foreskin in a long-term culture, we encountered an interesting phenomenon which was the conversion of the fibroblast dominant condition to epithelial-like tissue formation. However, the basic mechanism(s) which may be involved in this conversion is not clear. This study was designed to evaluate the cells protein secretion activity and examine...

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ژورنال

عنوان ژورنال: Reproduction

سال: 2001

ISSN: 1470-1626,1741-7899

DOI: 10.1530/rep.0.1220657